Services
Proteomics

i3S Proteomics Core Facility provides investigators access to the analysis of protein samples from solutions, bands or protein spots from 1D or 2D SDS gels.

i3S/Ipatimup is a node of the Portuguese Mass Spectrometry network - RNEM. Its members are Hugo OsórioHugo Osório (RNEM node director and member of the managing team) and Celso ReisCelso Reis (member of RNEM implementation team).

 

The proteomics unit performs the following analyses:

1. Mass Analysis of Intact Proteins, Peptides and Metabolites by mass spectrometry.

2. Protein identification by Peptide Mass Fingerprint - PMF (MALDI-TOF).

3. Protein identification by PMF following peptide sequencing/fragmentation (MALDI-TOF/TOF, PMF+MS/MS).

4. Protein Post-translational characterization.

The mass spectrometry equipment, 4800 Plus MALDI TOF/TOF Analyzer (AB SCIEX), is optimized to perform the aforementioned studies.

 

 

The services include:

 

Enzymatic digestion and peptide extraction of isolated proteins from 1D or 2D SDS-PAGE gels or purified proteins in solution.

Peptide analysis by mass spectrometry (MALDI-TOF) with the option of peptide sequencing by MS/MS (MALDI-TOF/TOF).

Protein search and identification using UnitProt, NCBI, or other protein sequence databases.

Delivery of a report with the identified protein(s), peptides, related genes, molecular weight and isoelectric point.

For sample preparation guidelines, click here.

For pricing, click here.

To submit samples you have to download our submission form, sign it, and send it to us by Fax or e-mail.

Click here, to obtain additional information about the methods used in this service.

For further details please contact: proteomics@ipatimup.pt

 

 
 
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